【Yeastern】YB Rapid Ligation Kit
YB Rapid Ligation Kit
FYC003-100R (100 rxns)
γT4 DNA Ligase 100 μl (3 U/μl)
10× Ligation Buffer A 200 μl
10× Ligation Buffer B 200 μl
Storage Buffer
Tris-HCl (pH7.5) 20 mM
KCl 50 mM
DTT 1 mM
EDTA 0.1 mM
Glycerol stabilizers 50%
10× Ligation Buffer A
Tris-HCl 0.4 mM
MgCl₂ 0.1 mM
DTT 0.1 mM
ATP (pH5.0 at 25°C) 5 mM
The performance of this buffer depends on the integrity of ATP. Store the buffer in small aliquots at -20°C to minimize degradation of ATP and DTT
10× Ligation Buffer B
Buffer contains an enhancer which dramatically increases ligation efficiency for blunt end DNA
Description
Yeastern’s yT4 DNA ligase catalyzes the joining of two strands of DNA between the 5’ – phosphate and the 3’ – hydroxyl groups of adjacent nucleotides in either a cohesive-ended or blunt-ended termini. The enzyme also repairs single-strand nicks in duplex DNA, RNA or DNA/RNA hybrids. YB Rapid Ligation Kit is designed for efficient ligation of DNA inserts into vectors in just 5 minutes.
Features
• Either sticky- or blunt-end, ligation can be achieved within 5 minutes
• Reaction mixture can be used directly for bacterial transformation
Applications
• Join double-strand DNA with cohesive or blunt termini
• Join oligonucleotide linkers to DNA sequence with blunt end
• Repair nicks in duplex DNA, RNA, or DNA-RNA hybrids
Combo Products
Quality Control
Undetectable endo-deoxyribonuclease and exo-deoxyribonuclease activities, undetectable ribonuclease activity, undetectable degradation of labeled oligonucleotide, passed the test of the capability to join cohesive- and blunt ended DNA fragments.
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