【Yeastern】T&A™ Cloning Kit, T&A™ Cloning Kit II

FYC001-20P (20 preps)

T&A™ Cloning Kit
T&A™ Cloning Vector 40 μl (25 ng/μl)
Control Insert DNA 10 μl (10 ng/μl)
γT4 DNA Ligase 20 μl
10× Ligation Buffer A 50 μl
10× Ligation Buffer B 50 μl
Forward Primer (M13-F) 50 μl (10 μM)
Reverse Primer (M13-R) 50 μl (10 μM)

FYC002-20P (20 preps)

T&A™ Cloning Vector 40 μl (25 ng/μl)
Control Insert DNA 10 μl (10 ng/μl)

FYC101-20P (20 preps)

T&A™ Cloning Kit II
T&A™ Cloning Vector II 40 μl (25 ng/μl)
Control Insert DNA 10 μl (10 ng/μl)
γT4 DNA Ligase 20 μl (2 U/μl)
10× Ligation Buffer A 50 μl
10× Ligation Buffer B 50 μl
Forward Primer (M13-F) 50 μl (10 μM)
Reverse Primer (M13-R) 50 μl (10 μM)

FYC102-20P (20 preps)

T&A™ Cloning Vector II 40 μl (25 ng/μl)
Control Insert DNA 10 μl (10 ng/μl)

Molecular cloning assisted by vectors is the most popular and common method to obtain genes of interest. Yeastern Biotech’s T&A™ Cloning Kit offers a quick, reliable and efficient method for cloning a variety of DNA sequences.

The T&A™ Cloning Kit (FYC001-20P) contains the T&A™ Cloning Vector and all the reagents needed for ligation. It is a convenient pack for cloning PCR product generated using thermostable DNA polymerases, such as YEAtaq DNA polymerase, which add a single terminal 3’-dA nucleotide overhang. After ligation, the mixture can be used directly for transformation into competent cells (ECOS™) or be purified first to achieve higher transformation efficiency.

Recently, YB has designed a new cloning vector for user convenience. The new T&A™ Cloning Vector II (FYC101-20P) consist of 2 EcoR I cutting sites (441, 500) within the multiple cloning site.

• Fast ligation, completed in only 5 minutes

• High transformation efficiency

• More accurate results

• Accept a wide range of inserts with different sizes

• Two types of ligation buffers provided for your convenience

• Allow blue/white screening

• Contain ampicillin marker for antibiotic selection

• Include M13 primer sites for convenient sequencing

Cloning of terminal 3’-dA nucleotides overhang PCR products up to 5 kb

• DNA concentration of the vectors is 25 ng/μl

• The absorbance ratio (A260/A280) is between 1.6~2.0

• The size of the vectors is about 2.7 kb

• The colony number of background control is less than 50 when the transformation efficiency of competent cells is 1 × 10⁸ cfu/μg DNA

• The colony number ratio of self-ligation control to positive control is less than 15%

• The colony number of positive control is more than 500 when the transformation efficiency of competent cells is 5 × 10⁸ cfu/μg DNA

• The ligation correctness with the control insert into the vectors is more than 87.5%

▍▏Map and Sequence reference points of the T&A™ Cloning Vector

* Before the insert incorporate into the T&A™ Cloning Vector, there is only one Hind III site and no Bgl II site. After the incorporation, the T and A nucleotide on the insert will complement the sequence on the vector and generate these two new sites. This merit of T&A™ Cloning Vector makes cloning more economic and convenient.

▍▏Map and Sequence reference points of the T&A™ Cloning Vector II

* Before the insert incorporate into the T&A™ Cloning Vector II, there is only one Hind III site and no Bgl II site. After the incorporation, the T and A nucleotide on the insert will complement the sequence on the vector and generate these two new sites. This merit of T&A™ Cloning Vector II makes cloning more economic and convenient.