【Yeastern】ECOS™ 9-5 Competent Cells(Strain: JM109)
ECOS™ 9-5 Competent Cells
FYE707-10VL (10 preps)
Efficiency > 5 x 10⁷ cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10⁻⁴ μg/μl)
FYE707-80VL (80 preps)
Efficiency > 5 x 10⁷ cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10⁻⁴ μg/μl)
FYE708-10VL (10 preps)
Efficiency > 1 x 10⁸ cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10⁻⁴ μg/μl)
FYE708-80VL (80 preps)
Efficiency > 1 x 10⁸ cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10⁻⁴ μg/μl)
FYE709-10VL (10 preps)
Efficiency > 1 x 10⁹ cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10⁻⁴ μg/μl)
FYE709-80VL (80 preps)
Efficiency > 1 x 10⁹ cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10⁻⁴ μg/μl)
FYE707-96WL (96 preps)
Efficiency > 5 x 10⁷ cfu/μg
50 μl/well 96 wells
FYE708-96WL (96 preps)
Efficiency > 1 x 10⁸ cfu/μg
50 μl/well 96 wells
FYE709-96WL (96preps)
Efficiency > 1 x 10⁹ cfu/μg
50 μl/vial 96 wells
Do not store the cells in liquid nitrogen!
Store at -70℃
Description
JM109, a K strain bacterium, carries mutations in recA and endA, leading to minimal recombination and improved quality of isolated plasmid DNA. In addition, the cells carry the F’ episome which allows blue/white screening for recombinant DNA. Thus, it has been a popular strain for routine subcloning. The cells can also be utilized for single-stranded DNA rescue when M13 or phagemid systems are used simultaneously. The JM109 strain is sensitive to all common antibiotics.
This product can become a powerful cloning tool when it is used in combination with Yeastern’s rapid ligation kit (#FYC003-100R). The ligation can be finished within 5 minutes and followed by 1-minute transformation. The fast growth rate of JM109 allows colonies to show up within 8 hours. Try this strain when you are in urgent.
Genotype
F' traD36 proA+ proB+ lacIq Δ(lacZ)M15 Δ(lac-proAB) supE44 hsdR17 recA1 gyrA96 thi¯1 endA1 relA1 e14¯ λ¯
Features
• Rapid growing strain
• Carries F' episome
Applications
• Preparation of ssDNA
• Construction of gene library
• Subcloning
Efficiency
> 1 x 108 cfu/μg
Recommended for:
• High efficiency cloning
• Cloning low abundance transcripts
• General cloning
• Gene library
> 1 x 109 cfu/μg
Recommended for:
• High efficiency cloning
• Cloning low abundance transcripts
• General cloning
• Gene library
> 5 x 107 cfu/μg
Recommended for:
• Subcloning
• General cloning
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