【Yeastern】YEAtaq DNA Polymerase

FYT001-500U (500 units)

YEataq DNA Polymerase (2.5 U/μl) 100 μl
10× Reaction Buffer 2 ml
dNTPs Mix (10 mM) 200 μl

FYT011-500U (500 units)

YEataq DNA Polymerase (2.5 U/μl) 100 μl
10× Reaction Buffer 2 ml

Storage Buffer
50 mM Tris-HCl (pH 9.0), 100 mM NaCl,
0.1 mM EDTA, 1% Triton X-100, 5 mM DTT,
50% Glycerol, Stabilizers

10× Reaction Buffer*
100 mM KCl, 20 mM MgSO₄•7H₂O, 200 mM
Tris-HCl (pH 8.8), 1% Triton X-100, 100 mM
(NH₄)₂SO₄, 1 mg/ml BSA

The reaction buffer is supplied as a 10× concentrate and should be diluted for use.

YEAtaq DNA Polymerase is a thermostable enzyme derived from the thermophilic bacterium Thermus aquaticus. It is able to withstand repeated heating to 95°C without significant loss of activity. The enzyme catalyzes 5’→3’ synthesis of DNA, has no detectable proofreading 3’→5’ exonuclease activity and possesses low 5’→3’ exonuclease activity. It also exhibits deoxynucleotidyl transferase activity, which normally results in the addition of extra adenines at the 3’-end of PCR products, required for the DNA ligation to TA vector.

Yeastern Biotech offers YEAtaq DNA Polymerase in two different packages: one with dNTPs mix and the other without.

The enzyme is in a recombinant form expressed in E. coli.

• Thermostable : half life is more than 40 min at 95°C.

•10× Reaction Buffer is supplied with two components: KCl and (NH₄)₂SO₄, the latter allows for PCR at wide range of magnesium concentrations and decreases non-specific priming.

• Incorporates modified nucleotides (e.g., dUTP, dITP, biotin-, digoxigenin-, fluorescently-labeled nucleotides).

• The error rate of YEAtaq DNA Polymerase in PCR is 2×10^-5 errors per nucleotide per cycle.

• Cloning

• Screening

• Primer extension

• Terminal dA tailing

• Routine PCR amplification of DNA fragments up to 3 Kb

• DNA labeling

• DNA sequencing

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 72°C.

• Nuclease activity is not detected after incubation of 1 μg λ/Hind III DNA with 5 units of YEAtaq DNA polymerase in 50 μl reaction buffer for 18 hours at 37°C.

• The absence of endo-, exodeoxyribonucleases and ribonucleases is confirmed by appropriate tests. Functional test is performed by PCR.

Some applications in which this product can be used may be covered by patents issued and applicable in certain countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a patent license depending upon the particular application and country in which the product is used.