【Yeastern】Total RNA Kit (Tissue and Plant) 2.0
Total RNA Kit (Tissue) 2.0
FYG307-100 (100 preps)
TR Buffer 45 ml
W1 Buffer 45 ml
W2 Buffer 15 ml
Elution Buffer 10 ml
DNase Incubation Buffer 10 ml
RZ Column 100 pcs
Collection Tube 100 pcs
FYG307-300 (300 preps)
TR Buffer 125 ml
W1 Buffer 125 ml
W2 Buffer 25 ml x2
Elution Buffer 30 ml
DNase Incubation Buffer 30 ml
RZ Column 300 pcs
Collection Tube 300 pcs
Description
The Total RNA Kit (Tissue) 2.0 is designed specifically for purifying total RNA from ffresh animal tissue or paraffin-embedded tissue. Detergents and a chaotropic salt are used to lyse cells and inactivate RNase. RNA in the chaotropic salt is bound to the glass fiber matrix of the spin column and once any contaminants have been removed using the Wash Buffer, the purified total RNA is eluted by the Elution Buffer.
Features
• Format : spin column (centrifuge)
• Yield : Up to 30 µg
Sample
30 mg of fresh animal tissue, 25 mg of paraffin-embedded tissue.
Applications
RT-PCR, Northern blotting, primer extension, mRNA selection cDNA synthesis.
Total RNA Kit (Plant) 2.0
FYG308-100 (100 preps)
PR Buffer 110 ml
W1 Buffer 45 ml
W2 Buffer 15 ml
Elution Buffer 10 ml
RZ Column 100 pcs
Collection Tube 100 pcs
FYG308-300 (300 preps)
PR Buffer 105 ml x3
W1 Buffer 125 ml
W2 Buffer 25 ml x2
Elution Buffer 30 ml
RZ Column 300 pcs
Collection Tube 300 pcs
Description
The Total RNA Kit (Plant) 2.0 is designed specifically for purifying total RNA from plant tissue or dry plant tissue. Detergents and a chaotropic salt are used to lyse cells and inactivate RNase. RNA in the chaotropic salt is bound to the glass fiber matrix of the spin column and once any contaminants have been removed using the Wash Buffer, the purified total RNA is eluted by the Elution Buffer.
Features
• Format : spin column (centrifuge)
• Yield : Up to 30 µg
Sample
100 mg of plant Tissue, 25 mg of dry plant Tissue
Applications
RT-PCR, Northern blotting, primer extension, mRNA selection cDNA synthesis.
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