【Yeastern】UniversAll™ Tissue Extraction/PCR Kits
UniversAll™ Tissue Extraction/PCR Kits
# FYU001-100P (100 preps)
UniversAll™ Extraction Buffer 5 ml
Primer U18S-F (10 μM) 20 μl
Primer U18S-R (10 μM) 20 μl
Primer P18S-F (10 μM) 20 μl
Primer P18S-R (10 μM) 20 μl
10× PCR Reaction Buffer (with 20 mM Mg2+) 500 μl
dNTPs Mix (10 mM) 50 μl
Uti DNA Polymerase (5 U/μl) 50 μl
Extraction Buffer Enhancer 250 μl
# FYU002-5ML
UniversAll™ Extraction Buffer 5 ml
Extraction Buffer Enhancer 250 μl
# FYU003-20ML
UniversAll™ Extraction Buffer for Animal Tissue 20 ml
Description
The UniversAll™ Tissue PCR Kits contain all the reagents necessary to rapidly extract genomic DNA from a wide range of biological samples prior to amplifying targets of interest by PCR. Genomic DNA is extracted from a small amount of samples simply by incubation in UniversAll™ Extract buffer for 10 minutes at 95 °C. The UniversAll™ Tissue PCR Kits offer a novel one-step UniversAll™ Extract buffer that eliminates the need for freezing cells or tissues with liquid nitrogen, mechanical disruption, organic extraction, column DNA purification, or alcohol precipitation.
Features
• Simple: single-step extraction of genomic DNA prior to PCR. No phenol/ chloroform.
• Fast: cells or tissues to PCR in 10 minutes.
• Convenient: includes our superior PCR enzymes and buffers for amplification directly from the extract for your convenience.
Applications
• Gene cloning
• Small or large scale PCR-based genotyping
• Traditional PCR; real-time PCR
• Suitable for rapid forensic and disease detection and diagnosis
Quality Control
Each lot of extraction buffer was tested for its extraction capability of standard animal and plant tissues for PCR and real-time PCR. It also has to pass the stability test before shipping.
Results
Mouse Tissue
PCR analysis of genomic DNA extracted from ICR mouse blood and tail with a U18S primer set.
Human Tissue
PCR analysis of genomic DNA extracted from human oral mucosal cells and human hair with a β-GBN primer set.
Paraffin Sample
Genomic DNA extracted from paraffin embeded mouse liver with a β-GBN primer set.
Real-time Quantitative PCR
Real-time quantitative PCR analysis of E. coli plasmid
DNA extracted by UniversAll™ extraction buffer.
点在看，传递你的品味
