AAT Bioquest®：细胞增殖与周期相关产品
▍▏细胞增殖- CytoTell™
比 CFSE 灵敏、方便、反应快；第一代与第二代无间隙；毒性低；多种颜色选择可用于 GFP 细胞和 FITC 标记的细胞。
Product Name |
Cat. # |
Ex/Em（nm） |
Size |
CytoTell™ Blue |
22251 |
403/454 |
500tests |
CytoTell™ Green |
22253 |
511/525 |
500tests |
CytoTell™ Orange |
22257 |
542/556 |
500tests |
CytoTell™ Red |
22255 |
628/643 |
500tests |
Cell tracking assays using CytoTell™ Green (Cat# 22253) and CFSE (Cat# 22022). Jurkat cells (~2x106 cells/mL) were stained with CytoTell™ Green or CFSE (0.5 μM) on Day 0. The cells were passed serially at 1:1 ratio for 9 days. Fluorescence intensity was measured with FACSCalibur™ flow cytometer (BD, San Jose, CA) using FL1 channel on the day after passage. Successive generations were represented by different colors.
▍▏活细胞周期检测试剂盒
Cell Meter™ 活细胞周期检测试剂盒，利用 AAT 特有的荧光探针，可进入活细胞核内与双链 DNA 结合发出荧光，无需固定，流式检测，操作简便。
Product Name |
Cat. # |
Ex/Em（nm） |
Size |
Cell Meter™ Fluorimetric Live Cell Cycle Assay Kit |
22841（流式） |
503/526（绿色） |
100tests |
22845 |
401/459 |
100tests |
|
22860 |
488/615（红色） |
100tests |
DNA profile in growing Jurkat cells. Jurkat cells were stained with cell Meter™ Fluorimet- ric Cell Cycle Assay Kit Optimized for 405 nm Violet Laser Excitation (Cat# 22845) for 30 minutes. The fluorescence intensity was measured using ACEA NovoCyte flow cytometer with the channel of Pacific Blue. In growing Jurkat cells, G0/G1 and G2/M phase histogram peaks are separated by the S-phase distribution.
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