AAT Bioquest®：专业荧光探针及相关检测试剂盒生产商（一）

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AAT Bioquest 20650 20651 20653 20700 20701 20702 20720 20721 20722 21130 21131 20510 20511 20512 20530 20531 20532 21080 21081 21082 21083 36310 36317 36318 36319 36200 36201 36202 36314 36315 36316 36370 36371 36373 36374 36379 36380 36381

第二信使

▍▏钙离子探针 ---- 信噪比最高胞内留存最好的荧光探针 Calbryte™

Calbryte™比 Cal® 系列有更好的胞内留存特性，比 Fluo3/4 以及 Fluo8 荧光强度更强，背景更低

Carbachol dose response was measured in CHO-M1 cells with Calbryte™ 520 AM and Fluo-4 AM. CHO-M1 cells were seeded overnight at 50,000 cells/100 μL/well in a 96-well black wall/clear bottom costar plate. 100 μL of 10 μg/ml Calbryte™ 520 AM in HH Buffer or 10 μg/ml Fluo-4 in HH Buffer was added and incubated for 45 minutes at 37 °C. Dye loading solution was then removed and replaced with 200 μL HH Buffer/well. Carbachol (50 μL/well) was added by FlexStation 3 to achieve the final indicated concentrations.

U2OS cells were seeded overnight at 40,000 cells per 100 μL per well in a Costar 96-well black wall/clear bottom plate. The growth medium was removed, and the cells were incubated with 100 μL of 4 μM Fluo-3 AM, Fluo-4 AM and Fluo-8® AM (Cat# 21080) in HHBS at 37 °C for 1 hour. The cells were washed twice with 200 μL HHBS, then imaged with a fluorescence microscope using FITC channel.

Product Name	Cat. #	Ex/Em（nm）
Calbryte™ 520 AM	20650/20651/20653	492/514（绿色）
Calbryte™ 590 AM	20700/20701/20702	573/588（橘红）
Calbryte™ 630 AM	20720/20721/20722	608/626（红色）
Cal-520®, AM	21130/21131	492/514（绿色）
Cal-590®, AM	20510/20511/20512	573/588（橘红）
Cal-630, AM	20530/20531/20532	608/626（红色）
Fluo-8®, AM	21080/21081/21082/21083	494/517（绿色）

细胞流式法检测钙流

Product Name	Cat. #	Ex/Em（nm）	Size
Cell Meter™ Flow Cytometric Calcium Assay Kit	36310	490/525 (绿色)	200test

高通量筛选，不含 Probenecid，无需洗涤

Product Name	Cat. #	Ex/Em（nm）
Screen Quest™ Calbryte-520 Probenecid-Free and Wash-Free Calcium Assay Kit	36317/36318/36319	492/514（绿色）
Screen Quest™ Calbryte-590 Probenecid-Free and Wash-Free Calcium Assay Kit	36200/36201/36202	573/588（橘色）
Screen Quest™ Fluo-8 No Wash Calcium Assay Kit	36314/36315/36316	490/525（绿色）

cAMP 检测试剂盒 --- 最便捷的检测方法

Product Name	Cat. #	优势
Screen Quest™ Colorimetric ELISA cAMP Assay Kit	36370/36371（比色）	只需一步清洗，无需乙酰化
Screen Quest™ Fluorimetric ELISA cAMP Assay Kit	36373/36374（荧光）	只需一步清洗，无需乙酰化
Screen Quest™ FRET No Wash cAMP Assay Kit	36379/36380/36381	无需清洗和乙酰化，适合 HTS